Journal: BMC Microbiology

Article Title: Influence of hydrological conditions on the Escherichia coli population structure in the water of a creek on a rural watershed

doi: 10.1186/1471-2180-10-222

Figure Lengend Snippet: E. coli enumeration in creek water according to land use in the watershed, and hydrological parameters.

Article Snippet: The E. coli CIP 7624 (ATCC 25922) was taken as the quality control strain.

Techniques: Sampling

Journal: BMC Microbiology

Article Title: Influence of hydrological conditions on the Escherichia coli population structure in the water of a creek on a rural watershed

doi: 10.1186/1471-2180-10-222

Figure Lengend Snippet: Structure and antibiotic resistance of the E. coli population in the stream during different hydrological conditions (χ 2 test P < 0.001 ***α = 0.01).

Article Snippet: The E. coli CIP 7624 (ATCC 25922) was taken as the quality control strain.

Techniques:

Journal: BMC Microbiology

Article Title: Influence of hydrological conditions on the Escherichia coli population structure in the water of a creek on a rural watershed

doi: 10.1186/1471-2180-10-222

Figure Lengend Snippet: Influence of a rain event during a wet period on E. coli density . The arrow indicates the beginning of 14 mm rain event.

Article Snippet: The E. coli CIP 7624 (ATCC 25922) was taken as the quality control strain.

Techniques:

Journal: BMC Microbiology

Article Title: Influence of hydrological conditions on the Escherichia coli population structure in the water of a creek on a rural watershed

doi: 10.1186/1471-2180-10-222

Figure Lengend Snippet: Structure and antibiotic resistance of the E. coli population in the stream in response to a rain event (χ 2 test P < 0.001 ***α = 0.01).

Article Snippet: The E. coli CIP 7624 (ATCC 25922) was taken as the quality control strain.

Techniques:

Journal: BMC Microbiology

Article Title: Influence of hydrological conditions on the Escherichia coli population structure in the water of a creek on a rural watershed

doi: 10.1186/1471-2180-10-222

Figure Lengend Snippet: Epidemiological types of E. coli B1 strains recovered from creek water.

Article Snippet: The E. coli CIP 7624 (ATCC 25922) was taken as the quality control strain.

Techniques:

Journal: BMC Microbiology

Article Title: Influence of hydrological conditions on the Escherichia coli population structure in the water of a creek on a rural watershed

doi: 10.1186/1471-2180-10-222

Figure Lengend Snippet: Distribution of E. coli B1 epidemiological types in relation to hydrological conditions (A) and before and after a rain event during a wet period (B) .

Article Snippet: The E. coli CIP 7624 (ATCC 25922) was taken as the quality control strain.

Techniques:

Journal: Carcinogenesis

Article Title: Muc1 knockout potentiates murine lung carcinogenesis involving an epiregulin-mediated EGFR activation feedback loop

doi: 10.1093/carcin/bgx039

Figure Lengend Snippet: MUC1 is expressed and contributes to EREG expression in fibroblasts. (A) Normal lung tissues from seven control WT and seven Muc1 KO mice and tumor tissues from seven NNK-treated WT and seven Muc1 KO mice were used to determine EREG concentration by ELISA assay. Ten micrograms of total protein from each sample was loaded to each well. Data shown are mean ± S.D; **P < 0.01. (B) Left, HFL-1 cells were treated with CSE (0, 10 and 20 µg/ml TPM) overnight. MUC1 expression was detected by Western blot. β-Actin was detected as an input control. Middle, HFL-1 cells were treated with CSE (20 µg/ml TPM) for the indicated time points. MUC1 mRNA level was detected by RT-PCR. β-Actin was detected as an input control. Right, HFL-1 cells were transfected with MUC1 siRNA or negative control siRNA for 48 h. MUC1 expression was detected by Western blot with antibody Muc1 GP1.4 against the extracellular domain (MUC1-N) and antibody MUC1 Ab-5 recognizing the C-terminal domain (MUC1-CT). β-Actin was detected as an input control. (C) HFL-1 and Hs888Lu cells were transfected with MUC1 siRNA or negative control siRNA for 24 h, before the cells were treated with CSE (20 µg/ml TPM), TNF-α (10 ng/ml), IL-6 (10 ng/ml) and IL-1β (10 ng/ml) overnight. EREG expression was detected by Western blot. β-Actin was detected as an input control. The intensity of the individual bands was quantified and normalized to the corresponding input control bands. Fold changes were calculated with the control taken as 1.

Article Snippet: The human lung cancer cell line A549, human fetal lung fibroblast line HFL-1, and human adult lung fibroblast line Hs888Lu were purchased from American Type Culture Collection (ATCC, Manassas, VA).

Techniques: Expressing, Concentration Assay, Enzyme-linked Immunosorbent Assay, Western Blot, Reverse Transcription Polymerase Chain Reaction, Transfection, Negative Control